Cryogenic light microscopy is an important part a correlative imaging workflow, allowing confirmation of localised molecules of interest prior to processing for downstream analysis such ultrastructural or compositional assessment or in short: It is a key mapping step.
Here, the authors describe adaptation of a standard upright widefield microscope into a cryogenic 3D laser-free confocal system using the Linkam CMS196. We demonstrate the necessary sample preparation steps followed by confocal imaging of biological cells and tissues.
Access the full version here: https://analyticalscience.wiley.com/do/10.1002/was.00170086
Nizamudeen, Zubair Ahmed, et al. "Adaptive 3D cryogenic correlative light imaging of native biology using laser free confocal system. Wiley Analytical Science
